An Optimized O9-1/Hydrogel System for Studying Mechanical Signals in Neural Crest Cells
2.7K Views
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11:02 min
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August 13th, 2021
DOI :
August 13th, 2021
•Tram P. Le*1, Xiaolei Zhao*1, Shannon Erhardt1,2, Jianhua Gu3, Huie Wang3, Tina O. Findley1, Jun Wang1,2
1Department of Pediatrics, McGovern Medical School, The University of Texas Health Science Center at Houston, 2The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences, The University of Texas Health Science Center at Houston, 3SEM AFM Core in the Houston Methodist Hospital Research Institute
Chapters in this video
0:05
Introduction
0:34
Preparation of Aminosilane-Coated and Siliconized Glass Coverslips
3:13
Preparation of Hydrogels
6:40
Molecular Analysis of Stiffness via Immunofluorescence Staining
8:04
Results: Characterization of O9-1 Cells Grown on Control and Modified Hydrogel System
10:31
Conclusion
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