An Automated Microscopic Scoring Method for the γ-H2AX Foci Assay in Human Peripheral Blood Lymphocytes
4.7K Views
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08:23 min
•
December 25th, 2021
DOI :
December 25th, 2021
•Shankari Nair1, Samantha Cairncross2, Xanthene Miles1, Monique Engelbrecht3, Peter du Plessis1, Julie Bolcaen1, Randall Fisher1, Roya Ndimba1, Charnay Cunningham4, Wilner Martínez-López5, Christian Schunck6, Charlot Vandevoorde1
1Radiation Biophysics Division, Nuclear Medicine Department, iThemba LABS, 2Department of Biotechnology, University of the Western Cape, 3Department of Medical Biosciences, University of the Western Cape, 4Division of Medical Physiology, Department of Medicine and Health Sciences, Stellenbosch University, 5Biodosimetry Service, Instituto de Investigaciones Biológicas Clemente Estable and Academic Unit on Radiation Protection, Faculty of Medicine, University of the Republic, 6MetaSystems Hard & Software GmbH
Chapters in this video
0:00
Introduction
0:44
Preparation of Samples
1:26
Sample Irradiations
1:35
Slide Preparation
2:17
Fixation and Immunofluorescence γ-H2AX Staining
3:44
Automated Metafer Scanning and Scoring
7:29
Results
8:03
Conclusion
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