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In Vitro Three-Dimensional Sprouting Assay of Angiogenesis Using Mouse Embryonic Stem Cells for Vascular Disease Modeling and Drug Testing

2.7K Views

08:04 min

May 11th, 2021

DOI :

10.3791/62554-v

May 11th, 2021


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In Vitro

Chapters in this video

0:04

Introduction

1:14

Embryoid Bodies (EB) Formation in Hanging Drop

3:02

Floating EBs Culture for Vascular Differentiation

3:41

3D Sprouting Angiogenesis Assay and Immunofluorescence Staining

5:44

Results: Microscopic Analysis and Quantification of Mouse EBs Undergoing Angiogenesis Assay

7:29

Conclusion

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