Large-Scale Preparation of Synovial Fluid Mesenchymal Stem Cell-Derived Exosomes by 3D Bioreactor Culture
4.1K Views
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09:48 min
•
July 26th, 2022
DOI :
July 26th, 2022
•1Department of Orthopedics, The First affiliated hospital of Shenzhen University, Shenzhen Second People's Hospital, 2Guangdong Provincial Research Center for Artificial Intelligence and Digital Orthopedic Technology, Shenzhen Second People's Hospital, 3Department of Child and Adolescent Psychiatry, Shenzhen Kangning Hospital, Shenzhen Mental Health Center, Shenzhen Key Laboratory for Psychological Healthcare & Shenzhen Institute of Mental Health
Chapters in this video
0:05
Introduction
0:40
Human Synovial Fluid Mesenchymal Stem Cells (SF-MSCs) Culture and Identification
2:42
Microcarrier Preparation and Perfusion Bioreactor
4:18
Exosome Identification and Safety Detection
5:01
Nanoparticle Tracking Analysis (NTA) and Western Blotting
6:48
Results: Identification and Analysis of SF-MSCs and the Exosomes
8:55
Conclusion
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