Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera (Hübner)
4.3K Views
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06:37 min
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July 1st, 2021
DOI :
July 1st, 2021
•1School of Chemical and Environmental Engineering, China University of Mining and Technology, 2State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 3Guangdong Laboratory of Lingnan Modern Agriculture, Shenzhen; Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences
Chapters in this video
0:05
Introduction
0:29
Design of Gene-Specific Primers and Preparation of sgRNA and Embryos
2:18
Microinjection of Embryos
3:47
Post-Injection Insect Rearing
4:41
Knock-Out Mutation Detection
5:27
Results: Selection and Synthesis of Target sgRNAs and Detection of Mutants by PCR and Gel Electrophoresis
6:08
Conclusion
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