Exploring Adipose Tissue Structure by Methylsalicylate Clearing and 3D Imaging
7.4K Views
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10:10 min
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August 19th, 2020
DOI :
August 19th, 2020
•Jérôme Gilleron1, Cindy Meziat1, André Sulen2, Stoyan Ivanov3, Jennifer Jager1, David Estève4, Catherine Muller4, Jean-Francois Tanti1, Mireille Cormont1
1Université Côte d'Azur, Inserm UMR1065, C3M, Team "Cellular and Molecular Pathophysiology of Obesity", Nice, France, 2Integrated Cardio Metabolic Center, Department of Medicine, Karolinska Institutet, Stockholm, Sweden, 3Université Côte d'Azur, Inserm UMR1065, C3M, Team "Haematometabolism in Diseases", Nice, France, 4Institut de Pharmacologie et de Biologie Structurale (IPBS), Université de Toulouse, CNRS, UPS, Toulouse, France
Chapters in this video
0:04
Introduction
0:35
Mouse and Human White Adipose Tissue Fixation, Permeabilization, and Saturation
2:59
Mouse and Human White Adipose Tissue Staining
5:28
Mouse and Human White Adipose Tissue Clearing
6:17
3D Confocal Imaging
7:23
3D Adipose Tissue Image Quantitative Result Extraction
7:57
Results: Representative Adipose Tissue Labeling and Analysis
9:34
Conclusion
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