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Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A)

5.5K Views

09:02 min

June 10th, 2020

DOI :

10.3791/61138-v

June 10th, 2020


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Mass Spectrometry

Chapters in this video

0:04

Introduction

0:44

In Vivo Ubiquitylation Assays Using pQCXIP-EYFP-CENP-A

2:19

Mass Spectrometry to Identify the Ubiquitylation Site of the EYFP-CENP-A K124R Mutant

4:55

Data Analysis

6:22

Results: EYFP-CENP-A K124 Mutant is Ubiquitylated and Interacts with HJURP

8:12

Conclusion

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