Whole-Mount In Situ Hybridization in Zebrafish Embryos and Tube Formation Assay in iPSC-ECs to Study the Role of Endoglin in Vascular Development
7.7K Views
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08:27 min
•
May 28th, 2020
DOI :
May 28th, 2020
•1Department of Physiology and Department of Cardiology of the Second Affiliated Hospital, Zhejiang University School of Medicine, 2Department of Cell Biology, State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, 3Wuxi Lung Transplant Center, Wuxi People's Hospital affiliated to Nanjing Medical University
Chapters in this video
0:04
Introduction
1:00
Zebrafish Embryo Collection and Fixation
2:55
Amplification and Microscopy
4:27
Tube Formation Assay
6:40
Results: Defective Tube Formation After Endoglin Knockdown
7:43
Conclusion
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