A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos
8.8K Views
•
08:55 min
•
January 14th, 2020
DOI :
January 14th, 2020
•Sanat Upadhyay1, Leoncio Vergara2, Pranjali Shah2, Jan-Åke Gustafsson3,4, Ioannis Kakadiaris1,4, Maria Bondesson5
1Computational Biomedicine Lab, Texas Institute of Measurement Evaluation and Statistics, University of Houston, 2Institute of Biosciences and Technology, Texas A&M Health Science Center, 3Department of Biology and Biochemistry, Center for Nuclear Receptors and Cell Signaling, University of Houston, 4Department of Biosciences and Nutrition, Novum, Karolinska Institutet, 5Department of Intelligent Systems Engineering, Indiana University
Chapters in this video
0:04
Title
0:41
Fish Husbandry and Preparation of Solutions and Embryos
1:44
Mounting in Agarose and Optimization of Agarose Solution
3:38
Time-lapse Imaging
6:07
Results: Extended Time-lapse Imaging of Zebrafish Embryos
7:54
Conclusion
Related Videos
14:29
Capturing Tissue Repair in Zebrafish Larvae with Time-lapse Brightfield Stereomicroscopy
10.7K Views
11:39
Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos
7.6K Views
10:05
Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning
10.7K Views
07:29
A Submerged Filter Paper Sandwich for Long-term Ex Ovo Time-lapse Imaging of Early Chick Embryos
22.1K Views
06:54
An Ex Vivo Method for Time-Lapse Imaging of Cultured Rat Mesenteric Microvascular Networks
6.8K Views
06:55
A Versatile Mounting Method for Long Term Imaging of Zebrafish Development
11.3K Views
08:01
Induction of Hypoxia in Living Frog and Zebrafish Embryos
9.7K Views
10:13
Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos
7.5K Views
09:42
Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos
9.5K Views
08:42
Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution
13.0K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved