Semi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence
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10:29 min
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August 14th, 2019
DOI :
August 14th, 2019
•Jessica Garcia1,2,3, David Barthelemy1,2,3, Florence Geiguer1,2, Julie Ballandier1,2,3, Kathryn W. Li4, Jean-Phillippe Aurel4, Frédérique Le Breton5, Claire Rodriguez-Lafrasse1, Brigitte Manship3, Sébastien Couraud6,7, Léa Payen1,2,3
1Laboratoire de Biochimie et Biologie Moléculaire, Groupe Hospitalier Sud, Hospices Civils de Lyon, 2Circulating Cancer (CIRCAN) Program, Hospices Civils de Lyon Cancer Institute, 3University of Lyon, Claude Bernard University, Cancer Research Center of Lyon, 4Biolidics Limited, 5Institut de Pathologie Multisites des HCL-Site Sud, Hospices Civils de Lyon, 6Acute Respiratory Disease and Thoracic Oncology Department, Lyon Sud Hospital, Hospices Civils de Lyon Cancer Institute, 7EMR-3738 Therapeutic Targeting in Oncology, Lyon Sud Medical Faculty, Lyon 1 University
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Chapters in this video
0:04
Title
1:11
Pre-Analytical Circulating Tumor Cells (CTC) Enrichment from Patient Whole Blood Samples
3:10
Spiral Microfluidic Device CTC Enrichment
4:20
Immunofluorescence (IF) Staining
6:32
IF Image Acquisition and Analysis
8:07
Results: Representative CTC Characterization and Enumeration
9:56
Conclusion
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