Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
8.5K Views
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12:15 min
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May 29th, 2019
DOI :
May 29th, 2019
•Tiago Franco de Oliveira*1,2, Antonio Anax Falcão de Oliveira*1, Miriam Lemos3, Mariana Veras3, Paulo Hilário Nascimento Saldiva3,4, Marisa Helena Gennari de Medeiros5, Paolo Di Mascio5, Ana Paula de Melo Loureiro1
1Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, 2Departamento de Farmacociências, Universidade Federal de Ciências da Saúde de Porto Alegre, 3Laboratório de Poluição Atmosfêrica Experimental - LIM05, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo, 4Instituto de Estudos Avançados, Universidade de São Paulo, 5Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo
Chapters in this video
0:04
Title
1:20
DNA Extraction
4:18
Quantification by HPLC-UV and Solid Phase Extraction for Analyses
6:20
Quantification by HPLC-ESI-MS/MS
9:19
Quantification of the DNA Lesions
10:36
Results: HPLC-UV and HPLC-ESI-MS/MS Analyses
11:25
Conclusion
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