Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors
6.2K Views
•
10:17 min
•
April 13th, 2019
DOI :
April 13th, 2019
•Chapters in this video
0:04
Title
1:12
Cell Culture
1:53
Measurement of Fluorescence and Limits of Detection
2:46
Detection and Quantification of 2 -FL in a Producer Strain
4:58
Selective Removal of Lactose: Treatment with Commercial Purified β-galactosidase
6:04
Selective Removal of Lactose: Pre-treatment with LacZ+ Strain
6:48
Selective Removal of Lactose: Lactose Precipitation with Ethanol
7:41
Results: Analysis of Fucosylated Human Milk Trisaccharides Using Genetically Encoded Biosensors
9:37
Conclusion
Related Videos
12:21
Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram
15.0K Views
06:54
Dissection of Human Retina and RPE-Choroid for Proteomic Analysis
10.8K Views
09:02
Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment
19.6K Views
11:08
Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
9.5K Views
07:57
Sampling and Pretreatment of Tooth Enamel Carbonate for Stable Carbon and Oxygen Isotope Analysis
13.8K Views
08:22
Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software
7.2K Views
10:15
Proteomic Analysis of Human Macrophage Polarization Under a Low Oxygen Environment
7.9K Views
09:16
Rapid Fluorescence-based Characterization of Single Extracellular Vesicles in Human Blood with Nanoparticle-tracking Analysis
9.7K Views
08:59
Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase
11.1K Views
06:13
Stepwise Dosing Protocol for Increased Throughput in Label-Free Impedance-Based GPCR Assays
6.5K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved