Simultaneous Quantification of Anti-vector and Anti-transgene-Specific CD8+ T Cells Via MHC I Tetramer Staining After Vaccination with a Viral Vector
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08:10 min
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November 28th, 2018
DOI :
November 28th, 2018
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Chapters in this video
0:04
Title
0:49
Sample Collection
1:21
Staining Set-up
1:59
Tetramer Staining and Preparation of Antibodies
3:17
Staining of Samples
4:24
Lysis of Erythrocytes
5:09
Flow Cytometric Measurement and Analysis
6:53
Results: Quantification of Antigen-specific CD8+ T Cells in Mouse Blood of Lymphoid Organs
7:36
Conclusion
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