The authors are most grateful to Thomas Wacker for his technical support. We further thank Dr. Mirjam Roth for her kind support.

The handling of the rats and the experimental procedures were conducted in accordance with European, national, and institutional guidelines for animal care.
1. Acclimatization and Habituation
NOTE: If rats were not transported, the acclimatization and habituation time can be reduced. For noninvasive identification, color the tail with skin-friendly marker.
<ol>
	<li>Week 1: Acclimatization 
	NOTE: Two sessions should be performed during the first week, one on the day of the arrival and a second one 2 days after arrival.
	<ol>
		<li>On the day of arrival of the rats:
		<ol>
			<li>Transfer the rats in pairs of two to their new home cage. Transfer some of the old nesting and bedding material as well as some of their familiar food pellets to facilitate the habituation to the new environment.</li>
			<li>Avoid lifting the rat by its tail. If possible, put one hand under the belly or around the chest and the other hand supporting the hind legs of the rats to lift them (Figure 1)26.</li>
		</ol>
		</li>
		<li>Two days after the day of arrival:</li>
		<li>Open the home cage of the rats and put a hand in the cage. Let the rats sniff and explore the hand. If the rats are trying to get out of the cage, hinder them by gently pressing them back in the cage. Continue with this procedure for the next four minutes.</li>
		<li>If the rats are afraid and do not start exploring their environment, repeat the procedure once per day until the rats start to explore. In this case, a habituation to the clicker might be necessary. 
		NOTE: Rats explore new things with all of their senses, so after some time, they will recognize the same experimenter as being familiar. It might happen that the rat does oral explorations of the hands, but it should not be in an invasive manner. If this situation occurs, the hand must be slowly taken away to ensure that the gloves are not destroyed.</li>
		<li>Add small pieces of the intended food reward to the home cage. 
		NOTE: The food reward must be in accordance with the requirements for laboratory animal nutrition. In this protocol, white chocolate is used (held at the end of a syringe), but other food, such as dry fruits, fresh fruits or cereals, is possible. If the animal facility hygiene requirements demand special conditions, there are various suppliers for treats for laboratory animals. The healthiest option is preferable, but a mix of high fat and high sugar may give the animals a greater motivation to participate.</li>
	</ol>
	</li>
	<li>Week 2: Habituation to the scientist and the food reward
	<ol>
		<li>Continue with the habitation to the hand and the food reward started in the previous week. Limit the interaction time to two minutes per day.</li>
		<li>In cases where the rat is very anxious, habituation to the clicker might be necessary.
		<ol>
			<li>To evaluate this behavior, click once every five seconds after the session on day 1. If the rat reacts with a fear-related behavior, the clicker must be habituated.</li>
			<li>For habituation to the clicker, click five times while the rat consumes the reward that is placed in the cage after the session. The habituation to the clicker is completed as soon as the rat no longer shows any fear-related behavior.</li>
		</ol>
		</li>
	</ol>
	</li>
	<li>Week 3: Habituation to intense handling
	<ol>
		<li>On days one to three, start with lifting or stroking the rat for several seconds and slowly extend the time (max. two minutes per rat). Stop if the rat shows any aversive signs such as freezing, dodging or hiding.</li>
		<li>On days four and five, end the handling in tickling, as described in Cloutier et al. (2012).27 
		NOTE: Indicators for a successful habituation are, for example, the rat taking the food reward if it is offered by hand or the rats getting close to the hand, exploring it, or touching it.</li>
	</ol>
	</li>
</ol>
2. Clicker Training
NOTE: A training session takes four minutes and consists of intervals of 30 seconds of training followed by 15 seconds of break. Remove any added material during the pause. The observer rat is free to do what it wants, and it is allowed to participate and to consume the reward, but the focus is solely on the other rat. All rats were tickled on day one and day five. For the training success, it is crucial that the experimenter pays attention to the animals&#39; behavior and is acting in a calm manner. The training strongly depends on the experimenter&#39;s abilities of timing and power of observation. No verbal feedback occurs in the training, and verbal communication with the animals may just occur outside the sessions. An indicator for the learning of the desired behavior is the repetition of the behavior.
<ol>
	<li>Preparation
	<ol>
		<li>Prepare the required material, such as the reward, the clicker/target stick combination, a timer, a clean cage, the metal tube, and any other specific training material.</li>
		<li>Transfer the home cage to a quiet place.</li>
		<li>Remove all items from the cage. Only the two rats (trained and observer) and the bedding material should remain in the cage.</li>
	</ol>
	</li>
	<li>Training day 1: Establishing a connection between the primary and secondary reinforcer
	<ol>
		<li>Place a short tunnel in the home cage, preferably one the rat is familiar with.</li>
		<li>Wait until the rat sits in the tunnel and then feed the rat with the reward. 
		NOTE: If the rat is not feeding under the circumstances of the training situation, the habituation phase must be extended or adjusted.</li>
		<li>As soon as the rat shows interest in the reward and is about to start nibbling on the reward, click and then let the rat feed on the reward. To ensure that the pairing of the two reinforces is successful, repeat clicking and then rewarding for 15 seconds. Do this at least twice during this first training session.</li>
		<li>If the rat did participate, check for signs of the establishment of a connection between the primary and the secondary reinforcer. The main criterion for this classification is the estimation of the food reward, which is shown by the rat by sniffing, looking around and at the hand that presents the food reward or lifting to its hind limbs in order to look for the reward (Figure 2). If this occurs, move on with the next part; otherwise, repeat &#34;Training day 1&#34;</li>
	</ol>
	</li>
	<li>Training days 2 to 5: Passing through a short tunnel 
	NOTE: A training session consists of alternating phases of 30 seconds of training and 15 seconds of break for four minutes.
	<ol>
		<li>Place a short tunnel of the same material as that of the first used/original tunnel that will link the two cages in the home cage.</li>
		<li>Start the timer and immediately begin with the first 30 seconds of training.
		<ol>
			<li>The first time the rat enters the tunnel, present the reward at the end of the tunnel. As soon as the rat enters the tunnel, click and let the rat eat the reward.</li>
			<li>Take away the food reward and wait until the rat shows interest for the tunnel once more. Click as soon as the rat enters the tunnel and present the reward at the end of the tunnel (Figure 3).</li>
			<li>After 30 seconds of training, add a 15 second break (remove the tunnel).</li>
		</ol>
		</li>
		<li>Repeat the 30 seconds of training with a subsequent 15 second break for four minutes.</li>
		<li>If the rat shows the desired behavior more than four times a minute, stop clicking and just reward with the primary reinforcer.</li>
		<li>Stop after four minutes.</li>
	</ol>
	</li>
	<li>Training week 2: Touching a target stick
	<ol>
		<li>Start the timer and immediately begin with the first 30 seconds of training. Add a break every 15 seconds and then go on with the training.</li>
		<li>Place the globe of the target stick near the rat.</li>
		<li>As soon as the rat shows interest in the globe, click and reward the rat. Repeat this several times.</li>
		<li>If the rat shows interest in the globe more than four times a minute, the next behavior to reinforce is &#34;touching the globe with the nose&#34;.</li>
		<li>If the rat repeats that behavior for more than four times a minute, stop clicking and just reward with the primary reinforcer.</li>
		<li>Stop after four minutes.</li>
	</ol>
	</li>
	<li>Training week 3 (five days) and week 4 (three days): Changing the cage through a tunnel 
	NOTE: Here, only passing through the tunnel towards the clean cage is reinforced. The training in week 4 has been shortened to three training days. This time is already sufficient for training success and gives the opportunity for one additional day for evaluation or data recording for further experiments.
	<ol>
		<li>Build up a connection between the home cage and a new clean cage by a tunnel by inserting a longer version of the tunnel already familiar to the rat.</li>
		<li>On day one, perform no training, and just let the rats inspect the tunnel. There is a good chance that they will even transfer to it on their own.</li>
		<li>On day two, give the rat two minutes to transfer through the tunnel on its own from the dirty to the clean side. If the rat freely transfers through the tunnel, skip to step 2.5.8.</li>
		<li>Otherwise, start the first 30 second training session (alternate between 30 seconds of training and a 15 second break) for four minutes.</li>
		<li>Click and reward the rat as soon as it shows interest in the tunnel.</li>
		<li>Go on to click and reward the entrance into the tunnel.</li>
		<li>Go on to click and reward if the rat goes deeper in the tunnel.</li>
		<li>Continue in a similar manner until the rat runs through the whole tunnel. As soon as the rat starts peeking out at the end of the tunnel into the clean cage, present the globe of the target stick, click, and reward for touching the globe.</li>
		<li>At last, guide the rat to leave the tunnel. For this process, use the globe of the target stick. Present the globe in front of the tunnel and click and reward the rat as soon as it steps in the new cage and is touching the globe.</li>
		<li>Transfer the rat back to the &#34;dirty&#34; home cage. Put one hand under the belly or around the chest and the other hand supporting the hind legs of the rats to lift them (Figure 1). Present the globe of the target stick in the center of the clean cage. Click and reward as the rat runs through the tunnel and is touching the target stick (Figure 4).</li>
		<li>If the rat repeats the behavior for more than four times a minute, stop clicking and just reward with the primary reinforcer.</li>
		<li>Stop after four minutes.</li>
	</ol>
	</li>
</ol>

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The authors have nothing to disclose.

The protocol described above is a useful extended application of our previously described clicker training protocol for laboratory mice10. The implementation requires only minutes per day over a total of seven weeks, including acclimatization, habituation and clicker training. For feasibility, this protocol was limited to week days with sessions of approximately ten minutes per pair of rats. The protocol can appear to be time-consuming to establish in large rat colonies, but if the animals are des...

Routine procedures can cause stress in laboratory animals1,2,3,4. It has been shown that cage changing does increase cardiovascular parameters and general activity in rats4,5,6. Such stress responses can at least be partially due to the physical disturbance and handling associated with cage changing procedures rather than the new unfamiliar environment2,4. Of particular importance is the negative effect of stress on the well-being of the regarded animal7. Further, stress induces changes in behavior and in other body parameters, including the autonomic nervous system, the neuroendocrine system and the immune system. Therefore, stress is often referred to as a possible source of unexpected deviations across animal experiments and should be avoided as much as possible in high-quality animal research7,8. One way to reduce stress in laboratory animals is by training. Training animals can generally be a very helpful tool for laboratory animal management and is, in fact, demanded by EU Directive 2010/63/EU9. Training can serve as a form of enrichment and helps to prepare animals for experiments; thus, training contributes to sustaining and enhancing well-being in laboratory settings10,11,12. A possible training method is positive reinforcement training (PRT). PRT is a form of operant conditioning where a reward (e.g., a food reward), is linked with a desired behavior13. This form of training is already commonly used in laboratory settings for nonhuman primates in order to reduce stress and to enhance well-being and has gained popularity in various other animal species10,13,14,15,16. Training of voluntary movement is also frequently used for refining animal husbandry management17,18,19. PRT is not only a helpful tool for cooperation when working with animals; it likewise is generally beneficial for the animals&#39; well-being, regardless of whether the trained behavior is directly used20. The positive reinforcement training protocol described here aims to avoid any form of stress during routinely performed cage changes by giving the rat the opportunity to voluntarily participate in the procedure.
In addition to the possible stress for the animals, working processes in animal facilities always provide pitfalls for the maintenance of hygiene status, especially as the direct contact between laboratory animals and animal caretakers bears the risk of hygiene contamination. In animal facilities specializing in rodents, the transfer of the animals from dirty into clean cages provides a regular and high workload. This procedure usually includes direct contact between animals and humans and thus represents a hygienic risk factor due to the possible transfer of pathogens settled on the human skin21. Aside from transfer from other animal vectors, pathogenic and nonpathogenic organisms intrude animal facilities most frequently via humans22,23. As reduced health is associated with reduced well-being, even subclinical infections can be a cause for nonreproducible experimental results, and this should clearly be avoided24,25. On this account, training animals to voluntary change cages further enables the conduction of the cage change after the training with hardly any direct contact between animal and animal caretaker, thereby reducing a potential hygienic risk while performing experimental procedures.
Our protocol for training rats to voluntarily change cages can be a helpful tool for laboratory rat management, as it links the training as a form of cognitive enrichment with performance of routine work. Accordingly, our protocol is an animal-friendly procedure that helps to increase the well-being of rats in laboratory animal facilities. As the cage change after the training phase can be performed with hardly any direct contact between animal and animal caretaker, it might additionally help to maintain hygiene at a high level and therefore further improve animal research.

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using clicker training and social observation to teach rats to voluntarily change cages

Cage cleaning is a routinely performed husbandry procedure and is known to induce stress in laboratory rats. As stress can have a negative impact on well-being and can affect the comparability and reproducibility of research results, the amount of stress experienced by laboratory animals should be minimized and avoided when possible. Further, the direct contact between the rat and animal caretaker during the cage change bears hygiene risks and therefore possibly negatively impacts the well-being of the rats and the quality of the research. 
Our protocol aims to improve the routinely performed cage changing procedure. For this reason, we present a feasible protocol that enables rats to learn via clicker training and observation to voluntarily change to a clean cage. This training helps to reduce stress caused by the physical disturbance and handling associated with the cage changes and concurrently enables a reduction in direct contact between animal and animal caretaker after the training phase is completed.
The implementation of clicker training to rats is fast and easy. Rats are generally interested in the training and efficiently learn the desired behavior, which entails changing cages through a pipe. Even without training, the rats learn to perform the desired behavior by observation, as 80% of the observational learning group successfully changed cages when tested. The training further helps to establish a relationship of trust between trainer and animal. As hygiene and well-being are both very important in animal experiments, this protocol might also help to improve high-quality research.

The training was conducted on a cohort of ten female Lister hooded (LD) rats. Ten untrained but gently handled female LD rats served as a control group. Gentle handling means that the rats were only lifted by their body and not lifted at the base of the tail. To evaluate the learning by observation, we added one further group of 10 female LD rats, which were not trained but were cage-mates of the trained rats and were able to observe the training. All rats completed the acclimatization an...

Watch this Scientific Journal Video about Using Clicker Training and Social Observation to Teach Rats to Voluntarily Change Cages at JoVE.com

Using Clicker Training and Social Observation to Teach Rats to Voluntarily Change Cages

This protocol introduces a method of cage change for rats via clicker training. Rats learn the desired behavior not only by direct training but also by observational learning. The implementation of this fast and easy protocol might help to improve well-being and hygiene in rodent facilities.

Cage cleaning is a routinely performed husbandry procedure and is known to induce stress in laboratory rats. As stress can have a negative impact on ...

using-clicker-training-social-observation-to-teach-rats-to

Universidad de Cartagena UDEC

Research

JoVE Journal

Behavior

10.0K Views.  Johannes Gutenberg-University Mainz. This protocol introduces a method of cage change for rats via clicker training. Rats learn the desired behavior not only by direct training but also by observational learning. The implementation of this fast and easy protocol might help to improve well-being and hygiene in rodent facilities.

Video: Using Clicker Training and Social Observation to Teach Rats to Voluntarily Change Cages

Using Chronic Social Stress to Model Postpartum Depression in Lactating Rodents

Exposure to chronic stress is a reliable predictor of depressive disorders, and social stress is a common ethologically relevant stressor in both animals and humans. However, many animal models of depression were developed in males and are not applicable or effective in studies of postpartum females. Recent studies have reported significant effects of chronic social stress during lactation, an ethologically relevant and effective stressor, on maternal behavior, growth, and behavioral neuroendocrinology. This manuscript will describe this chronic social stress paradigm using repeated exposure of a lactating dam to a novel male intruder, and the assessment of the behavioral, physiological, and neuroendocrine effects of this model. Chronic social stress (CSS) is a valuable model for studying the effects of stress on the behavior and physiology of the dam as well as her offspring and future generations. The exposure of pups to CSS can also be used as an early life stress that has long term effects on behavior, physiology, and neuroendocrinology.

This article describes the use of chronic resident intruder social stress as an ethologically relevant paradigm to model postpartum depression and anxiety in lactating rodents.

Exposure to chronic stress is a reliable predictor of depressive disorders, and social stress is a common ethologically relevant stressor in both animals ...

Measuring Sensitivity to Viewpoint Change with and without Stereoscopic Cues

The speed and accuracy of object recognition is compromised by a change in viewpoint; demonstrating that human observers are sensitive to this transformation. Here we discuss a novel method for simulating the appearance of an object that has undergone a rotation-in-depth, and include an exposition of the differences between perspective and orthographic projections. Next we describe a method by which human sensitivity to rotation-in-depth can be measured. Finally we discuss an apparatus for creating a vivid percept of a 3-dimensional rotation-in-depth; the Wheatstone Eight Mirror Stereoscope. By doing so, we reveal a means by which to evaluate the role of stereoscopic cues in the discrimination of viewpoint rotated shapes and objects.

We discuss a novel method forviewpoint-rotation of visual stimuli, and demonstrate using a mirror stereoscopethe three-dimensional percept of rotation-in-depth. The technique can be used to investigate the role of stereoscopic cues in encoding viewpoint-rotated figures.

The speed and accuracy of object recognition is compromised by a change in viewpoint; demonstrating that human observers are sensitive to this ...

Uncovering Beat Deafness: Detecting Rhythm Disorders with Synchronized Finger Tapping and Perceptual Timing Tasks

A set of behavioral tasks for assessing perceptual and sensorimotor timing abilities in the general population (i.e., non-musicians) is presented here with the goal of uncovering rhythm disorders, such as beat deafness. Beat deafness is characterized by poor performance in perceiving durations in auditory rhythmic patterns or poor synchronization of movement with auditory rhythms (e.g., with musical beats). These tasks include the synchronization of finger tapping to the beat of simple and complex auditory stimuli and the detection of rhythmic irregularities (anisochrony detection task) embedded in the same stimuli. These tests, which are easy to administer, include an assessment of both perceptual and sensorimotor timing abilities under different conditions (e.g., beat rates and types of auditory material) and are based on the same auditory stimuli, ranging from a simple metronome to a complex musical excerpt. The analysis of synchronized tapping data is performed with circular statistics, which provide reliable measures of synchronization accuracy (e.g., the difference between the timing of the taps and the timing of the pacing stimuli) and consistency. Circular statistics on tapping data are particularly well-suited for detecting individual differences in the general population. Synchronized tapping and anisochrony detection are sensitive measures for identifying profiles of rhythm disorders and have been used with success to uncover cases of poor synchronization with spared perceptual timing. This systematic assessment of perceptual and sensorimotor timing can be extended to populations of patients with brain damage, neurodegenerative diseases (e.g., Parkinson&#8217;s disease), and developmental disorders (e.g., Attention Deficit Hyperactivity Disorder).

Behavioral tasks that allow for the assessment of perceptual and sensorimotor timing abilities in the general population (i.e., non-musicians) are presented. Synchronization of finger tapping to the beat of an auditory stimuli and detecting rhythmic irregularities provides a means of uncovering rhythm disorders.

A set of behavioral tasks for assessing perceptual and sensorimotor timing abilities in the general population (i.e., non-musicians) is presented here ...

Training Rats to Voluntarily Dive Underwater: Investigations of the Mammalian Diving Response

Underwater submergence produces autonomic changes that are observed in virtually all diving animals. This reflexly-induced response consists of apnea, a parasympathetically-induced bradycardia and a sympathetically-induced alteration of vascular resistance that maintains blood flow to the heart, brain and exercising muscles. While many of the metabolic and cardiorespiratory aspects of the diving response have been studied in marine animals, investigations of the central integrative aspects of this brainstem reflex have been relatively lacking. Because the physiology and neuroanatomy of the rat are well characterized, the rat can be used to help ascertain the central pathways of the mammalian diving response. Detailed instructions are provided on how to train rats to swim and voluntarily dive underwater through a 5 m long Plexiglas maze. Considerations regarding tank design and procedure room requirements are also given. The behavioral training is conducted in such a way as to reduce the stressfulness that could otherwise be associated with forced underwater submergence, thus minimizing activation of central stress pathways. The training procedures are not technically difficult, but they can be time-consuming. Since behavioral training of animals can only provide a model to be used with other experimental techniques, examples of how voluntarily diving rats have been used in conjunction with other physiological and neuroanatomical research techniques, and how the basic training procedures may need to be modified to accommodate these techniques, are also provided. These experiments show that voluntarily diving rats exhibit the same cardiorespiratory changes typically seen in other diving animals. The ease with which rats can be trained to voluntarily dive underwater, and the already available data from rats collected in other neurophysiological studies, makes voluntarily diving rats a good behavioral model to be used in studies investigating the central aspects of the mammalian diving response.

Detailed instructions are provided on how to train rats to voluntarily dive underwater through a 5 m long Plexiglas maze. Because the brains of rats have been very well characterized, voluntarily diving rats may help elucidate the central pathways of the mammalian diving response.

Underwater submergence produces autonomic changes that are observed in virtually all diving animals. This reflexly-induced response consists of apnea, a ...

A Method for Evaluating the Reinforcing Properties of Ethanol in Rats without Water Deprivation, Saccharin Fading or Extended Access Training

Operant oral self-administration methods are commonly used to study the reinforcing properties of ethanol in animals. However, the standard methods require saccharin/sucrose fading, water deprivation and/or extended training to initiate operant responding in rats. This paper describes a novel and efficient method to quickly initiate operant responding for ethanol that is convenient for experimenters and does not require water deprivation or saccharin/sucrose fading, thus eliminating the potential confound of using sweeteners in ethanol operant self-administration studies. With this method, Wistar rats typically acquire and maintain self-administration of a 20% ethanol solution in less than two weeks of training. Furthermore, blood ethanol concentrations and rewards are positively correlated for a 30 min self-administration session. Moreover, naltrexone, an FDA-approved medication for alcohol dependence that has been shown to suppress ethanol self-administration in rodents, dose-dependently decreases alcohol intake and motivation to consume alcohol for rats self-administering 20% ethanol, thus validating the use of this new method to study the reinforcing properties of alcohol in rats.

This protocol describes a novel and efficient method to quickly initiate operant responding for ethanol in rats that, contrary to standard methods, does not require water deprivation or saccharin/sucrose fading to initiate responding.

Operant oral self-administration methods are commonly used to study the reinforcing properties of ethanol in animals. However, the standard methods ...

Pavlovian Conditioned Approach Training in Rats

Cues that are contingently paired with unconditioned, rewarding stimuli can acquire rewarding properties themselves through a process known as the attribution of incentive salience, or the transformation of neutral stimuli into attractive, &#34;wanted&#39; stimuli capable of motivating behavior. Pavlovian conditioned approach (PCA) develops after the response-independent presentation of a conditioned stimulus (CS; e.g., a lever) that predicts the delivery of an unconditioned stimulus (US; e.g., a food pellet) and can be used to measure incentive salience. During training, three patterns of conditioned responses (CRs) can develop: sign-tracking behavior (CS-directed CR), goal-tracking behavior (US-directed CR), and an intermediate response (both CRs). Sign-trackers attribute incentive salience to reward-related cues and are more vulnerable to cue-induced reinstatement of drug-seeking as well as other addiction-related behaviors, making PCA a potentially valuable procedure for studying addiction vulnerability. Here, we describe materials and methods used to elicit PCA behavior from rats as well as analyze and interpret PCA behavior in individual experiments.

Here, we present a protocol to elicit Pavlovian conditioned approach behavior in rats. This procedure can be used to measure individual differences in the tendency to approach and attribute incentive salience to reward-related cues and investigate addiction vulnerability.

Cues that are contingently paired with unconditioned, rewarding stimuli can acquire rewarding properties themselves through a process known as the ...

Introducing Clicker Training as a Cognitive Enrichment for Laboratory Mice

Establishing new refinement strategies in laboratory animal science is a central goal in fulfilling the requirements of Directive 2010/63/EU. Previous research determined a profound impact of gentle handling protocols on the well-being of laboratory mice. By introducing clicker training to the keeping of mice, not only do we promote the amicable treatment of mice, but we also enable them to experience cognitive enrichment. Clicker training is a form of positive reinforcement training using a conditioned secondary reinforcer, the "click" sound of a clicker, which serves as a time bridge between the strengthened behavior and an upcoming reward. The effective implementation of the clicker training protocol with a cohort of 12 BALB/c inbred mice of each sex proved to be uncomplicated. The mice learned rather quickly when challenged with tasks of the clicker training protocol, and almost all trained mice overcame the challenges they were given (100% of female mice and 83% of male mice). This study has identified that clicker training for mice strongly correlates with reduced fear in the mice during human-mice interactions, as shown by reduced anxiety-related behaviors (e.g., defecation, vocalization, and urination) and fewer depression-like behaviors (e.g., floating). By developing a reliable protocol that can be easily integrated into the daily routine of the keeping of laboratory mice, the lifetime experience of welfare in the mice can be improved substantially.

The development of new refinement strategies for laboratory mice is a challenging task that contributes towards fulfilling the 3R principle. This protocol introduces clicker training as a cognitive enrichment program for laboratory mice.

Establishing new refinement strategies in laboratory animal science is a central goal in fulfilling the requirements of Directive 2010/63/EU. Previous ...

Integrating Computerized Linguistic and Social Network Analyses to Capture Addiction Recovery Capital in an Online Community

The article describes a new methodology designed with the aim of finding a comprehensive, unobtrusive, and accurate way of capturing social recovery capital development in online communities of recovery from alcohol and drug (AOD) addiction. Recovery capital was conceptualised as both engagement in the online recovery community and identification with the community. To measure recovery capital development, naturally occurring data were extracted from the social media page of a specific recovery program, with the page being set up as a resource for a face-to-face recovery program. To map engagement with the online community, social network analysis (SNA) capturing online social interaction was performed. Social interaction was measured through the linkages between the online contributors/members of the online community as represented by program clients, staff, and supporters from the broader community. To capture markers of social identification with the online community, computerised linguistic analysis of the textual data (content from posts and comments) was conducted. Recovery capital captured in this way was analysed against retention data (a proxy outcome indicator), as days spent in the (face-to-face) recovery program. The online data extracted was linked to participant data in regards to program retention to test prediction of a key recovery outcome. This approach allowed the examination of the role of online support communities and assessment of the association between recovery capital (developed via the online community of recovery) and recovery outcomes.

The article describes a novel approach for analyzing dynamic online social interactions (in an online context) exemplified by a study of an online community of recovery from alcohol and drug addiction.

The article describes a new methodology designed with the aim of finding a comprehensive, unobtrusive, and accurate way of capturing social recovery ...

Modified Blood Collection from Tail Veins of Non-anesthetized Mice with a Vacuum Blood Collection System and Eyeglass Magnifier

Blood sample collection is the basis of experimental animal research. It is of importance to obtain adequate blood samples for various research purposes. The tail veins of mice are small, and it is sometimes difficult to obtain the required blood volume by conventional puncture methods. This study investigates the superiority of repeated blood sample collection from tail veins of mice through use of a vacuum blood collection system and eyeglass magnifier (experimental group) compared to conventional blood sampling methods (conventional group), performed by beginners and experts, respectively. With the help of an eyeglass magnifier, a butterfly needle tip is inserted into the tail vein of each mouse in the experimental group. When the vein is penetrated successfully, a blood sample is collected in the vacuum collection tube by insertion of the rubber end of a butterfly needle into the vacuum blood collection tube. The plunger is then used to collect blood without the help of the eyeglass magnifier in the conventional group. Success rates of blood sample collection by the beginners and experts were shown to be 70% vs. 100% (p &#60; 0.01) in the experimental group and 35% vs. 85% (p &#60; 0.01) in the conventional group. For both beginners and experts, puncture times required for obtaining required blood sample were significantly lower in the experimental group compared to the conventional group (2.40 &#177; 0.75 vs. 2.90 &#177; 0.31, p &#60; 0.05; 1.15 &#177; 0.37 vs. 1.55 &#177; 0.76, p &#60; 0.05). In conclusion, the presented blood sampling technique is feasible and easy to practice and enables frequent sampling of adequate blood volumes from non-anesthetized mice.&#160;

This study reports blood sampling from tail vein in mice using a vacuum extraction tube system with eyeglass magnifier. Our method is easy to practice and could be used for repeat blood sampling in mice.

Blood sample collection is the basis of experimental animal research. It is of importance to obtain adequate blood samples for various research purposes. ...

Using Facial Electromyography to Assess Facial Muscle Reactions to Experienced and Observed Affective Touch in Humans

"Affective" touch is believed to be processed in a manner distinct from discriminatory touch and to involve activation of C-tactile (CT) afferent fibers. Touch that optimally activates CT fibers is consistently rated as hedonically pleasant. Patient groups with impaired social-emotional functioning also show disordered affective touch ratings. However, relying on self-reported ratings of touch has many limitations, including recall bias and communication barriers. Here, we describe a methodological approach to study affective responses to touch via facial electromyography (EMG) that circumvents the reliance on self-report ratings. Facial EMG is an objective, quantitative, and non-invasive method to measure facial muscle activity indicative of affective responses. Responses can be assessed across healthy and patient populations without the need for verbal communication. Here, we provide two separate datasets demonstrating that CT-optimal and non-optimal touch elicit distinct facial muscle reactions. Moreover, facial EMG responses are consistent across stimulus modalities, e.g. tactile (experienced touch) and visual (observed touch). Finally, the temporal resolution of facial EMG can detect responses on timescales that supersede that of verbal reporting. Together, our data suggest that facial EMG is a suitable methodology for use in affective tactile research that can be used to supplement, or in some cases, supplant, existing measures.

We describe a protocol to assess facial muscle activity in response to experienced and observed tactile stimulation using facial electromyography.

"Affective" touch is believed to be processed in a manner distinct from discriminatory touch and to involve activation of C-tactile (CT) afferent fibers. ...