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Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells

12.3K Views

12:06 min

January 11th, 2019

DOI :

10.3791/58297-v

January 11th, 2019


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Human Primordial Germ Cell like Cells

Chapters in this video

0:04

Title

1:01

Inoculation and Conversion of Primed Pluripotency Human Induced Pluripotent Stem Cells (hiPSCs)

3:40

Embryoid Body (EB) Formation Using Microwell Plates

5:52

Transfer of EBs to Low Attachment Plates and Generation of hPGCLCs on the Surface of EBs

8:00

Immunohistochemical Staining of EBs

9:41

Results: Successful Generation of EBs with hPGCLCs on their Surface

11:29

Conclusion

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