Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
32.2K Views
•
10:16 min
•
June 28th, 2018
DOI :
June 28th, 2018
•Stefan Schoenfelder*1, Biola-Maria Javierre*1,2, Mayra Furlan-Magaril1,3, Steven W. Wingett1,4, Peter Fraser1,5
1Nuclear Dynamics Programme, The Babraham Institute, Babraham Research Campus, 2IJC Building, Campus ICO-Germans Trias i Pujol, Josep Carreras Leukemia Research Institute, 3Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, 4Bioinformatics Group, The Babraham Institute, Babraham Research Campus, 5Department of Biological Science, Florida State University
Chapters in this video
0:04
Title
0:52
Formaldehyde Fixation
2:14
HindIII Digestion and Biotinylation of Restriction Fragment Overhangs
4:09
Hybrid In-solution Capture
6:53
Isolation of Promoter Fragment-containing Ligation Products
8:55
Results: IL8 PCHi-C Profile in Human Hematopoietic Cells
9:34
Conclusion
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