Quantification of Site-specific Protein Lysine Acetylation and Succinylation Stoichiometry Using Data-independent Acquisition Mass Spectrometry
11.5K Views
•
12:49 min
•
April 4th, 2018
DOI :
April 4th, 2018
•Chapters in this video
0:04
Title
1:18
Quantitatively Acetylate and/or Succinylate Proteins Using Isotope-labeled Acetic or Succinic Anhydride
3:48
Digest Reacted Protein Samples Using Glu-C Endoproteinase
6:27
Fractionate Peptides Using Offline Basic-pH Reversed Phase HPLC
8:15
Example Data Analysis Tutorial
10:28
Results: Succinylation Stoichiometry Ratio Calculation of Pre-succinylated BSA
11:13
Conclusion
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