Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer
12.5K Views
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09:52 min
•
April 26th, 2018
DOI :
April 26th, 2018
•1Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kindai University, 2Faculty of Biology-Oriented Science and Technology, Kindai University, 3Wellcome Trust/Cancer Research UK Gurdon Institute and Department of Zoology, University of Cambridge, 4Laboratory of Reproductive Biology, Graduate School of Agriculture, Kyoto University, 5Institute of Advanced Technology, Kindai University
Chapters in this video
0:05
Title
0:52
Preparation of Deionized Bovine Serum Albumin
1:48
Oocyte Collection
3:26
Preparation of Donor Cells for Nuclear Transfer and Enucleation of Oocytes
5:32
Fusion of Donor Cell and Enucleated Oocyte
7:42
Activation of Reconstructed Oocytes and Treatment with Trichostatin A and Vitamin C
8:26
Results: Somatic Cell Nuclear Transfer in Mice using Trichostatin A and Vitamin C Treatment
9:15
Conclusion
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