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Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

9.8K Views

10:21 min

February 21st, 2018

DOI :

10.3791/57033-v

February 21st, 2018


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Myeloid Precursor Cells

Chapters in this video

0:04

Title

0:58

Media Preparation

1:24

Production of Retrovirus

3:28

Tritation of Virus Containing a Puromycin Reporter

5:04

Transduction of 32D/G-CSF-R Cells

6:10

32D/G-CSF-R Cell Differentiation Assay

7:32

Results: Study of Proliferation and Differentiation of 32D/G-CSF-R Cells in Interleukin-3 and Granulocyte Colony Stimulating Factor Containing Media

9:21

Conclusion

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