Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry
7.5K Views
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11:20 min
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March 29th, 2018
DOI :
March 29th, 2018
•Claudia Siverino1, Barbara Tabisz2, Tessa Lühmann3, Lorenz Meinel3, Thomas Müller4, Heike Walles1,2, Joachim Nickel1,2
1Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik (IGB), Translationszentrum Würzburg 'Regenerative Therapien für Krebs- und Muskuloskelettale Erkrankung', Institutsteil Würzburg, 2Lehrstuhl für Tissue Engineering und Regenerative Medizin, Universitätsklinikum Würzburg, 3Lehrstuhl für Pharmazeutische Technologie und Biopharmazie, Universität Würzburg, 4Lehrstuhl für molekulare Pflanzenphysiologie und Biophysik, Julius-von-Sachs Institut für Biowissenschaften, Universität Würzburg
Chapters in this video
0:04
Title
0:46
Expression and Purification of BMP2-K3Plk
5:46
Covalent Coupling Technique of BMP2-K3Plk to Azide Functionalized Agarose Beads
7:04
Measuring Alkaline Phosphatase (ALP) Expression to Prove the In Vitro Bioactivity of the Produced BMP2-K3Plk Before and After the Coupling Reaction
8:34
Results: Evaluation of the Bioactivity of BMP2-K3Plk after Site-Directed Immobilization
9:54
Conclusion
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