Sign In

Sample Preparation for Endopeptidomic Analysis in Human Cerebrospinal Fluid

8.9K Views

10:23 min

December 4th, 2017

DOI :

10.3791/56244-v

December 4th, 2017


Explore More Videos

Endopeptidomic Analysis

Chapters in this video

0:05

Title

1:12

Pre-treatment of Bulk Volume CSF without Quantification

2:24

Pre-treatment of Small Volume CSF Samples with Isobaric Labeling

4:00

MWCO Filtration, Desalting, and Sample Clean-up by Solid Phase Extraction

6:00

Offline High-pH Reverse Phase HPLC Sample Fractionation

7:09

LC-MS Analysis

7:47

Results: Peptide Identification After LC-MS Analysis

9:13

Conclusion

Related Videos

article

16:57

MALDI Imaging Mass Spectrometry of Neuropeptides in Parkinson's Disease

25.9K Views

article

14:48

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

6.8K Views

article

10:51

Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting

16.0K Views

article

09:22

Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus

2.4K Views

article

10:36

High Resolution Quantitative Synaptic Proteome Profiling of Mouse Brain Regions After Auditory Discrimination Learning

10.4K Views

article

07:08

A High Throughput, Multiplexed and Targeted Proteomic CSF Assay to Quantify Neurodegenerative Biomarkers and Apolipoprotein E Isoforms Status

7.7K Views

article

09:20

Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue

27.5K Views

article

09:00

Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain

3.0K Views

article

07:55

Harnessing the Power of MicroRNA Cargoes in Small Extracellular Vesicles Released from Fresh-Frozen Human Brain Sections

342 Views

article

11:49

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue

4.4K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved