JoVE Logo

Sign In

PIP-on-a-chip: A Label-free Study of Protein-phosphoinositide Interactions

9.4K Views

10:58 min

July 27th, 2017

DOI :

10.3791/55869-v

July 27th, 2017


Explore More Videos

Protein phosphoinositide Interactions

Chapters in this video

0:05

Title

1:04

Fabricating Micropatterned PDMS Blocks

2:14

Preparing Small Unilamellar Vesicles (SUVs)

3:29

Assembling the Microfluidic Device

4:42

Forming Supported Lipid Bilayers (SLBs)

7:56

Testing PLC-δ1 PH Domain Interction with PI(4,5)P2-containing SLBs

8:49

Results: Assessing PLC-δ1 PH Domain: Phosphoinositide Interaction with PIP-on-a-chip Assay

9:56

Conclusion

Related Videos

article

07:26

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

9.5K Views

article

10:26

Biotinylated Cell-penetrating Peptides to Study Intracellular Protein-protein Interactions

9.4K Views

article

10:52

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation

10.4K Views

article

10:41

Capturing the Interaction Kinetics of an Ion Channel Protein with Small Molecules by the Bio-layer Interferometry Assay

8.2K Views

article

08:07

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

8.5K Views

article

08:49

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

3.9K Views

article

10:17

A Mass Spectrometry-Based Approach to Identify Phosphoprotein Phosphatases and their Interactors

2.4K Views

article

12:27

A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors

3.3K Views

article

09:40

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

15.1K Views

article

02:23

pH Modulation Assay on Supported Lipid Bilayers to Detect Protein-Phosphoinositide Interactions

101 Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved