Live-cell Measurement of Odorant Receptor Activation Using a Real-time cAMP Assay
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09:11 min
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October 2nd, 2017
DOI :
October 2nd, 2017
•1Department of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis of National Ministry of Education, Shanghai Jiao Tong University School of Medicine, 2Department of Molecular Genetics and Microbiology, Duke University Medical Center, 3Department of Neurobiology, Duke Institute for Brain Sciences, Duke University Medical Center, 4Institute of Health Science, Chinese Academy of Science/Shanghai Jiao Tong University School of Medicine
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Chapters in this video
0:05
Title
0:50
Plating and Transfection of Hana3A Cells
3:03
Transfection of Plasmids
5:24
Stimulation and Measuring OR Activity Using the Real-time cAMP Assay
7:27
Results: Kinetic Measurements of the Response of OR5AN1 to Muscone
8:23
Conclusion
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