Brain Membrane Fractionation: An Ex Vivo Approach to Assess Subsynaptic Protein Localization
10.3K Views
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09:49 min
•
May 12th, 2017
DOI :
May 12th, 2017
•1Unitat de Farmacologia, Departament Patologia i Terapèutica Experimental, Facultat de Medicina, IDIBELL, Universitat de Barcelona, L'Hospitalet de Llobregat, 2Institut de Neurociències, Universitat de Barcelona, 3Center for Neurosciences of Coimbra, Institute of Biochemistry, Faculty of Medicine, University of Coimbra
Chapters in this video
0:00
Title
0:18
Introduction
2:22
Obtaining Mouse Brain Synaptosomes Using a Discontinuous Sucrose Gradient
4:37
Pre-post and Extra-synaptic Fraction Isolation
6:52
Analyze Samples and Validate Fractionation
7:26
Representative Results
8:53
Conclusion
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