Recombinant α- β- and γ-Synucleins Stimulate Protein Phosphatase 2A Catalytic Subunit Activity in Cell Free Assays

0:33

Preparation of Buffers and Reagents

0:38

Preparing p-Nitrophenol Phosphate (pNPP) Buffer

1:21

Preparing Malachite Green Solution

2:21

Preparation of Activator

2:42

Preparing Malachite Green + Tween 20 (MGT) Working Solution

2:58

Preparing Threonine Phosphopeptide (pT)

3:31

Synuclein Preparation

3:59

Preparing Phosphate Standards

4:45

Phosphate Standards Step 2

5:17

PP2A Activity Assay

6:42

Photometric Analysis

6:53

Representative Results

9:01

Conclusion