Using X-ray Crystallography, Biophysics, and Functional Assays to Determine the Mechanisms Governing T-cell Receptor Recognition of Cancer Antigens
11.3K Views
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09:53 min
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February 6th, 2017
DOI :
February 6th, 2017
•Chapters in this video
0:05
Title
1:05
T-cell Receptor (TCR) and Human Leukocyte Antigen-resticted Tumor-derived Peptide (pHLA) Refolding and Purification by Fast Protein Liquid Chromatography (FPLC)
3:43
Surface Plasmon Resonance (SPR) Equilibrium Binding and Thermodynamic Analyses
5:55
Protein Crystallization and X-ray Diffraction
7:39
Results: Molecular Analysis of gp100280-288 Recognition by CD8+ T-cells
8:59
Conclusion
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