Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
9.6K Views
•
11:01 min
•
November 23rd, 2016
DOI :
November 23rd, 2016
•Chapters in this video
0:05
Title
1:11
Preparation of 2-pyridyl Undec-10-ynyl Carbonate and Undec-10-ynyl 2-oxopyridine 1-carboxylate
2:44
Preparation of Undec-10-ynyl-N-[(3S)-2-oxoazetidin-3-yl]carbamate
4:17
Click Chemistry with Azide-PEG3-Biotin
5:14
Removal of Excess Click Chemistry Reagents
6:29
Streptavidin Enrichment
7:57
Click Chemistry with Azide-PEG3-Fluor 545 on Tissue Slices
8:59
Results: Synthesis and Applications of NAAA Activity Based Probe, ARN14686
10:21
Conclusion
Related Videos
11:16
Hydrophobic Salt-modified Nafion for Enzyme Immobilization and Stabilization
16.1K Views
09:07
Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay
6.7K Views
16:33
Detection of Protein Palmitoylation in Cultured Hippocampal Neurons by Immunoprecipitation and Acyl-Biotin Exchange (ABE)
35.7K Views
07:37
Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening
1.8K Views
10:24
NMR-Based Activity Assays for Determining Compound Inhibition, IC50 Values, Artifactual Activity, and Whole-Cell Activity of Nucleoside Ribohydrolases
9.9K Views
12:47
Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources
9.2K Views
05:51
Direct Detection of the Acetate-forming Activity of the Enzyme Acetate Kinase
23.0K Views
10:16
In Vitro Enzyme Measurement to Test Pharmacological Chaperone Responsiveness in Fabry and Pompe Disease
8.0K Views
09:33
In Vitro Assay to Measure Phosphatidylethanolamine Methyltransferase Activity
9.8K Views
08:10
Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System
8.7K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved