Adenofection: A Method for Studying the Role of Molecular Chaperones in Cellular Morphodynamics by Depletion-Rescue Experiments
7.6K Views
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12:34 min
•
September 16th, 2016
DOI :
September 16th, 2016
•1Département de biologie moléculaire, biochimie médicale et pathologie, Faculté de médecine, Centre de recherche sur le cancer de l'Université Laval, 2Oncology, Centre de recherche du CHU de Québec, Université Laval, 3Laboratoire d'études moléculaires des valvulopathies (LEMV), Groupe de recherche en valvulopathies (GRV), Quebec Heart and Lung Institute/Research Center, 4Department of Surgery, Université Laval
Chapters in this video
0:05
Title
0:49
Plating HeLa-RFP-H2B Cells
3:38
Adenovirus Transduction and Endogenous Protein Knockdown by siRNA Transfection in HeLa-RFP-H2B Cells Using CaPi Precipitates
7:46
Live Cell Imaging of Mitotic Cells and Data Analysis
9:36
Results: Adenofection Effectively Introduces Proteins of Interest without Affecting Mitosis
11:44
Conclusion
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