Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
12.5K Views
•
10:49 min
•
September 20th, 2016
DOI :
September 20th, 2016
•Chapters in this video
0:05
Title
0:43
Preparation of Cultured Cells
1:23
Treatment of Cells and Photocrosslinking
4:34
Attachment of Fluorescent Tag by Click Chemistry for Visualization of Labeled Proteins
5:48
Attachment of Biotin Tag by Click Chemistry for Affinity Purification of Labeled Proteins
8:54
Results: Live-cell Photoaffinity Labeling Identifies a Major Itraconazole-binding Protein
10:14
Conclusion
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