JoVE Logo

Sign In

Novel Atomic Force Microscopy Based Biopanning for Isolation of Morphology Specific Reagents against TDP-43 Variants in Amyotrophic Lateral Sclerosis

8.7K Views

13:31 min

February 12th, 2015

DOI :

10.3791/52584-v

February 12th, 2015


Explore More Videos

Keywords Atomic Force Microscopy

Chapters in this video

0:05

Title

2:47

12 Rounds of Negative Panning Against Bovine Serum Albumin (BSA)

4:19

AFM Process

6:26

8 Rounds of Negative Panning Against Healthy Immunoprecipitated TDP-43

7:54

Positive Panning against Immunoprecipitated TDP-43 from Individuals with ALS

11:17

Results: Analysis of Potential ALS Clones

12:59

Conclusion

Related Videos

article

12:58

Characterizing Individual Protein Aggregates by Infrared Nanospectroscopy and Atomic Force Microscopy

9.7K Views

article

10:51

Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting

16.0K Views

article

09:31

Visualization of Amyloid β Deposits in the Human Brain with Matrix-assisted Laser Desorption/Ionization Imaging Mass Spectrometry

10.5K Views

article

08:33

Targeting Alpha Synuclein Aggregates in Cutaneous Peripheral Nerve Fibers by Free-floating Immunofluorescence Assay

8.2K Views

article

11:03

Use of Capillary Electrophoresis Immunoassay to Search for Potential Biomarkers of Amyotrophic Lateral Sclerosis in Human Platelets

7.2K Views

article

06:10

Visualizing Synaptic Degeneration in Adult Drosophila in Association with Neurodegeneration

6.1K Views

article

Nuclei Isolation and Super-Resolution Structured Illumination Microscopy for Examining Nucleoporin Alterations in Human Neurodegeneration

1.2K Views

article

08:59

Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis

2.5K Views

article

07:14

Optogenetic Phase Transition of TDP-43 in Spinal Motor Neurons of Zebrafish Larvae

5.9K Views

article

09:00

Biochemical Purification and Proteomic Characterization of Amyloid Fibril Cores from the Brain

3.0K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved