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Sample Preparation Strategies for Mass Spectrometry Imaging of 3D Cell Culture Models

18.1K Views

08:14 min

December 5th, 2014

DOI :

10.3791/52313-v

December 5th, 2014


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3D Cell Culture

Chapters in this video

0:05

Title

1:28

Three-dimensional Cell Culture and Preparation of Agarose Coated 96 Well Plates

2:32

Seed and Harvest the Three-dimensional Cultures and Embed in Gelatin

4:11

Slice and Thaw Mount the Gelatin-embedded Three-dimensional Cell Cultures for Mass Spectrometric Imaging

5:03

Matrix Application and Sample Preparation for MALDI Imaging

5:42

MALDI-MSI Analysis Using a MALDI-TOF System and Optional Data Analysis Methods

7:28

Results: Mass Spectrometry Imaging of Three-dimensional Cell Cultures

7:49

Conclusion

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