Rapid Fractionation and Isolation of Whole Blood Components in Samples Obtained from a Community-based Setting
16.0K Views
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11:31 min
•
November 30th, 2015
DOI :
November 30th, 2015
•Amy Weckle1,2, Allison E. Aiello3, Monica Uddin1,4, Sandro Galea5, Rebecca M. Coulborn6, Richelo Soliven7, Helen Meier6, Derek E. Wildman1,2
1Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, 2Department of Molecular and Integrative Physiology, University of Illinois at Urbana-Champaign, 3Department of Epidemiology, Gillings School of Global Public Health, University of North Carolina, 4Department of Psychology, University of Illinois at Urbana-Champaign, 5Department of Epidemiology, Mailman School of Public Health, Columbia University, 6Department of Epidemiology, University of Michigan School of Public Health, 7Center for Molecular Medicine and Genetics, Wayne State University School of Medicine
Chapters in this video
0:05
Title
2:09
Serum, DNA, and PBMC Isolation Stage 1
3:49
DNA Isolation Stage 1
4:36
Leukocyte RNA Isolation Stage 1
6:02
PBMC Isolation Stage 2
8:06
Serum Isolation Stage 2 and PBMC Isolation Stage 3
10:30
Results: Ideal Leukocyte RNA Processing Yields
10:50
Conclusion
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