Expression of Fluorescent Proteins in Branchiostoma lanceolatum by mRNA Injection into Unfertilized Oocytes
11.0K Views
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09:31 min
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January 12th, 2015
DOI :
January 12th, 2015
•Estelle Hirsinger1, João Emanuel Carvalho2, Christine Chevalier1,3, Georges Lutfalla4, Jean-François Nicolas1, Nadine Peyriéras5, Michael Schubert2
1Département de Biologie du Développement et Cellules Souches, Institut Pasteur, 2Laboratoire de Biologie du Développement de Villefranche-sur-Mer (UMR7009 CNRS/UPMC Univ Paris 06), Sorbonne Universités, 3Equipe Epigenetic Control of Normal and Pathological Hematopoiesis, Centre de Recherche en Cancérologie de Marseille, 4Unité de Dynamique des Interactions Membranaires Normales et Pathologiques, CNRS UMR5235/DAA/cc107/Université Montpellier II, 5Plateforme BioEmergences IBiSA FBI, CNRS-NED, Institut de Neurobiologie Alfred Fessard
Chapters in this video
0:05
Title
1:21
Poly-lysine Coated Dishes
2:04
Agarose Coated Dishes
2:38
Injection Mix and Loading Injection Needles
3:40
Sample Collection
4:38
Oocyte Injection
6:39
Fertilization, Selection, and Embryo Culture
7:55
Results: Expression of Fluorescent Proteins After mRNA Injection
8:56
Conclusion
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