Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix
27.5K Views
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09:00 min
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February 9th, 2015
DOI :
February 9th, 2015
•Nayeli Rodríguez-Fuentes1, Olivia Reynoso-Ducoing2, Ana Rodríguez-Hernández2, Javier R. Ambrosio-Hernández2, Maria C. Piña-Barba1, Armando Zepeda-Rodríguez3, Marco A. Cerbón-Cervantes4, José Tapia-Ramírez5, Luz E. Alcantara-Quintana6
1Depto. Materiales Metálicos y Cerámicos, Instituto de Investigaciones en Materiales, Universidad Nacional Autónoma de México (UNAM), 2Depto. of Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), 3Depto. Biología Celular y Tisular, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), 4Depto de Biología Celular, Facultad de Química, Universidad Nacional Autónoma de México (UNAM), 5Depto. Genetica y Biología Molecular, Cinvestav-IPN, 6Subd. de Investigación, Centro Nacional de la Transfusión Sanguínea, Secretaria de Salud
Chapters in this video
0:05
Title
1:15
Isolation of Human Mesenchymal Stem Cells
3:20
Expansion of Mesenchymal Stem Cells
4:37
Preparation of Porous Bone Matrix Disk and Culture of Human Mesenchymal Stem Cells
5:54
Cell Proliferation Assay and Measuring Colony Forming Units
6:56
Results: Characterization of Isolated Human Mesenchymal Stem Cells
8:30
Conclusion
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