High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
13.7K Views
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11:34 min
•
May 5th, 2014
DOI :
May 5th, 2014
•Marianne Lucas-Hourani1, Hélène Munier-Lehmann2, Olivier Helynck2, Anastassia Komarova1, Philippe Desprès3, Frédéric Tangy1, Pierre-Olivier Vidalain1
1Unité de Génomique Virale et Vaccination, Virology Department, Institut Pasteur, CNRS UMR3569, 2Unité de Chimie et Biocatalyse, Biochemistry and Structural Biology Department, Institut Pasteur, CNRS UMR3523, 3Unité des Interactions Moléculaires Flavivirus-Hôtes, Virology Department, Institut Pasteur
Chapters in this video
0:05
Title
1:34
Preparation of 96-well Daughter Plates with Compounds
3:03
Preparation of Cell Cultures to Determine Compound Toxicity
4:18
Preparation of Cell Cultures Infected by rMV2/Luc
5:37
Luciferase Activity Measurement
6:36
Secondary Screening for Toxicity and Broad-spectrum Antiviral Activity
8:52
Results: Identification of Anti-viral Hit Compounds
11:06
Conclusion
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