TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination
14.6K Views
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12:13 min
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October 8th, 2012
DOI :
October 8th, 2012
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Chapters in this video
0:05
Title
1:16
Preparation of Chitin Flakes
1:39
Oligonucleotide Design and Polymerase Chain Reaction
3:45
Chitin-induced Natural Transformation
4:59
Removal of Selective Cassette(s) by FLP-recombination
5:46
Plasmid Curing
6:21
Results: Representative TransFLP Modifications of V. cholerae
11:34
Conclusion
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