Chemotactic Response of Marine Micro-Organisms to Micro-Scale Nutrient Layers

0:16

Mask

0:55

Cleaning and baking the wafer

2:11

Coating the wafer

4:13

Soft baking the wafer

4:47

UV exposure

6:14

Developing the wafer

8:19

Application of PDMS to produce channels

12:44

Experiment 1: Investigating the chemotactic response of marine micr

13:44

Adding organisms and substrates to glass syringes

14:39

Assembling the microfluidics system on the microscope stage

15:53

Observation under microscope

16:37

Removing bubbles from channel

17:59

Visualization of nutrient gradient and microbes

18:42

Measurement of chemotaxis

19:47

Experiemnt 2: Investigating the effects of shear on marine bacteria