Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions
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14:04 min
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February 1st, 2010
DOI :
February 1st, 2010
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Chapters in this video
0:00
Title
1:22
Introduction
1:41
Selection of Bait and Appropriate MYTH System
3:04
Generation of tMYTH Baits by Gap Repair
3:07
a. Vector Preparation and Amplification of Gene of Interest
5:30
b. Yeast Transformation and Recovery of Bait Plasmid DNA
7:03
Bait Validation
8:10
MYTH Screening
8:12
a. Large Scale Yeast Transformation
11:31
b. Bait Dependency Test
12:54
Representative Results/ Outcome
13:12
Conclusion
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