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Measuring Plasma Membrane Protein Endocytic Rates by Reversible Biotinylation

16.9K Views

11:32 min

December 23rd, 2009

DOI :

10.3791/1669-v

December 23rd, 2009


Transcript

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Plasma Membrane Proteins

Chapters in this video

0:00

Title

0:56

Introduction

1:20

Cell Culture and Solution Preparation

3:37

Biotinylation, Quenching and Internalization of Proteins

7:30

Protein Assay, Affinity Chromatography and SDS-Page Analysis of Proteins

9:49

Dopamine Transporter Internalization

11:02

Conclusion

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