Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy
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10:59 min
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October 18th, 2024
DOI :
October 18th, 2024
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0:00
Introduction
1:59
Site‐Specific Dual‐End Labeling of RNA with Förster Resonance Energy Transfer (FRET) Fluorophores for Structural and Functional Studies
5:25
Preparation and Surface Treatment of Microfluidic Chambers for Total Internal Reflection Fluorescence (TIRF) Microscopy
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Phospholipid Vesicle Encapsulation for RNA Delivery and Single‐Molecule Förster Resonance Energy Transfer (smFRET)‐Total Internal Reflection Fluorescence (TIRF) Microscopy
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