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Split-Luciferase Reassembly Assay to Measure Endoplasmic Reticulum-Mitochondria Contacts in Live Cells

1.5K Views

09:09 min

October 11th, 2024

DOI :

10.3791/66862-v

October 11th, 2024


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Split Luciferase Assay

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0:00

Introduction

1:55

Maintaining and Seeding HEK293T Cells for Split‐Rluc Plasmid Transfection

3:38

Polyethyleneimine (PEI)‐Mediated HEK293T Cell Transfection and Post‐Transfection Cell Seeding

5:55

Chemical Treatment and Luciferase Assay to Measure the Level of ER‐Mitochondria Contacts in Live Cells

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