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Quantitative 3D Imaging of Trypanosoma cruzi-Infected Cells, Dormant Amastigotes, and T Cells in Intact Clarified Organs

3.0K Views

08:17 min

June 23rd, 2022

DOI :

10.3791/63919-v

June 23rd, 2022


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Quantitative 3D Imaging

Rozdziały w tym wideo

0:04

Introduction

1:10

Tissue Clearing and DNA Staining

2:40

Immunostaining

4:27

RI Matching and Mounting

5:22

Results I: Visualization of Trypanosoma cruzi‐Infected Cells, Dormant Amastigotes, and Inflammation Foci in Cleared Organs

6:27

Results II: Immunodetection of Vasculature in Cleared Trypanosoma cruzi‐Infected Organs

7:07

Conclusion

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