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De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

3.4K Views

08:23 min

February 18th, 2022

DOI :

10.3791/63366-v

February 18th, 2022


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Ribosome Profiling

Rozdziały w tym wideo

0:04

Introduction

1:01

Environment Setup and RiboCode Installation

1:23

Data Preparation

3:35

Removing Ribosomal RNA Contamination

3:59

Aligning the Clean Reads to the Genome

4:25

Size Selection of Ribosome Protected Fragments, Identification of P-Sites, and De novo Annotate Translating Open Reading Frames

4:59

Results: Identification of the Novel Open Reding Frames (ORFs) with the Application of RiboCode

7:20

Conclusion

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