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Monitoring Protein Aggregation Kinetics In Vivo using Automated Inclusion Counting in Caenorhabditis elegans

2.7K Views

06:49 min

December 17th, 2021

DOI :

10.3791/63365-v

December 17th, 2021


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Protein Aggregation

Rozdziały w tym wideo

0:04

Introduction

0:42

Growth of an Age-Synchronized Population of C. elegans

1:12

Sample Preparation of C. elegans in a Multiwell Plate

1:43

Image Acquisition on the High-Throughput Confocal Microscope

2:45

Automated Inclusion Counting Using CellProfiler

4:07

Global Fitting of Inclusion Count Data Using AmyloFit

5:39

Results: Fitting the Data to a Mathematical Model in AmyloFit

6:16

Conclusion

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