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Using 2-Photon Microscopy to Quantify the Effects of Chronic Unilateral Ureteral Obstruction on Glomerular Processes

2.2K Views

11:47 min

March 4th, 2022

DOI :

10.3791/63329-v

March 4th, 2022


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2 Photon Microscopy

Rozdziały w tym wideo

0:05

Introduction

0:55

Preparing the Munich Wistar Frmter or SD Rat for Unilateral Ureteral Obstruction (UUO) Surgery

1:53

Surgical Preparation for Intravital 2-Photon Imaging

4:04

Positioning the Rat for Imaging and Image Acquisition for Quantitative Analysis

6:16

Calculating Red Blood Cell Flow in Surface Glomerular Capillary Loops and Renal Vasculature Using a Linescan Function

7:45

Calculating White Blood Cell (WBC) Occlusion in Glomerular Capillary Loops

8:38

Results: Quantification of Glomerular Processes in the Unilateral Ureteral Obstruction (UUO) Model of Rats

10:56

Conclusion

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