Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry
Please note that all translations are automatically generated. Click here for the English version.
1.7K Views
•
09:41 min
•
December 28th, 2021
DOI :
December 28th, 2021
•Meng-Fei Xu1,2, Yu-Hao Yang1,2, Ya-Lan Wei3,4, Jing-Lian Zhang1,2, Xuan Lin1,2, Xiao-Yan Lin1,2, Hui Chen1,2, Zhen-Yu She1,2
1Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, 2Key Laboratory of Stem Cell Engineering and Regenerative Medicine, Fujian Province University, 3Fujian Obstetrics and Gynecology Hospital, 4Medical Research Center, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University
Rozdziały w tym wideo
0:05
Introduction
1:13
Construction of GSK923295-Mediated Centromere Protein E (CENP-E) Inhibition Mouse Models
2:18
Hematoxylin-eosin (HE) Staining and Histopathology
3:57
Immunofluorescence and Confocal Microscopy
5:31
Flow Cytometry
7:04
Results: Analysis of Seminiferous Tubules and the Disruption of Spermatogenesis Post CENP-E Inhibition by GSK923295
8:59
Conclusion
Powiązane Filmy
12:26
Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
18.5K Views
10:46
Motility of Single Molecules and Clusters of Bi-Directional Kinesin-5 Cin8 Purified from S. cerevisiae Cells
2.5K Views
08:16
Single-Molecule Analysis of Sf9 Purified Superprocessive Kinesin-3 Family Motors
1.8K Views
10:09
Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes
1.9K Views
09:41
Imaging Centrosomes in Fly Testes
15.9K Views
10:30
Cytological Analysis of Spermatogenesis: Live and Fixed Preparations of Drosophila Testes
26.1K Views
09:39
Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
15.0K Views
08:06
Identification of Kinesin-1 Cargos Using Fluorescence Microscopy
7.8K Views
05:35
Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
15.1K Views
09:02
Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A)
5.5K Views
Copyright © 2025 MyJoVE Corporation. Wszelkie prawa zastrzeżone