Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
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6.8K Views
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14:48 min
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May 8th, 2021
DOI :
May 8th, 2021
•Licia E. Prestagiacomo1, Caterina Gabriele1, Paola Morelli2, Maria Antonietta Rota3, Stefano Alba3, Giovanni Cuda1, Rocco Damiano4, Marco Gaspari1
1Research Centre for Advanced Biochemistry and Molecular Biology, Department of Experimental and Clinical Medicine, Magna Graecia University of Catanzaro, 2Department of Health Science, Magna Graecia University of Catanzaro, 3Romolo Hospital, 4Department of Experimental and Clinical Medicine, Magna Graecia University of Catanzaro
Rozdziały w tym wideo
0:00
Introduction
0:55
Protocol
1:25
Protocol: FASP Digestion
5:29
Protocol: SCX Purification
8:08
Protocol: Protein Quantifcation by External Standard
9:23
Protocol: NanoLC-MS/MS and DIA Analysis
11:31
Protocol: High-pH C18 Fractionation for Spectral Library
13:09
Protocol: Data Analysis
13:35
Expected Results
13:53
Conclusion
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