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Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection

4.2K Views

10:25 min

June 5th, 2021

DOI :

10.3791/62486-v

June 5th, 2021


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SARS CoV 2

Rozdziały w tym wideo

0:04

Introduction

0:53

Plating and Maintenance of Vero E6 Cells

2:22

Preparing the Vesicular Stomatitis Virus-Spike-EGFP (VSV-S-EGFP) Pseudovirus

3:46

Titering the VSV-S-eGFP Pseudovirus

5:52

Neutralizing SARS-CoV-2 Pseudovirus Using Convalescent Patient Serum and Commercial Antibodies

9:07

Results: SARS-CoV-2 Pseudovirus Neutralization by Commercial Antibodies and Convalescent Patient Serum

10:00

Conclusion

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