Identifying the Binding Proteins of Small Ligands with the Differential Radial Capillary Action of Ligand Assay (DRaCALA)
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3.4K Views
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09:26 min
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March 19th, 2021
DOI :
March 19th, 2021
•Rozdziały w tym wideo
0:05
Introduction
0:42
Preparation of Whole Cell Lysates
1:43
Nickel-Nitrilotriacetic Acid (Ni-NTA) Affinity Purification of Relseq and GppA
2:55
Gel Filtration
3:33
Synthesis of 32P-Labeled Guanosine Pentaphosphate (pppGpp) and Guanosine Tetraphosphate (ppGpp)
5:08
DRaCALA Screening of the Target Proteins of (p)ppGpp
6:37
Quantification and Identification of Potential Target Proteins
7:46
Results: DRaCALA Plate Screening and Quantification
8:39
Conclusion
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