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Segmenting Growth of Endothelial Cells in 6-Well Plates on an Orbital Shaker for Mechanobiological Studies

4.9K Views

12:28 min

June 3rd, 2021

DOI :

10.3791/61817-v

June 3rd, 2021


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Endothelial Cells

Rozdziały w tym wideo

0:00

Introduction

1:33

Fabrication of Stainless-steel Module

1:43

3D Printing of Polydimethylsiloxane (PDMS) Mold

2:39

Casting of PDMS Ring

3:41

Preparation of 1% Pluronic F-127

4:32

Coating of a 6-well Plate

8:20

Seeding of Endothelial Cells

8:58

Shear Stress Application using an Orbital Shaker

9:57

Representative Results

11:31

Conclusion

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